نشریه ارمغان دانش
سال بیست و ششم شماره 1 (پیاپی 144، فروردین و اردیبهشت 1400)

Using in vitro MTT assay and Real-time PCR, the cytotoxicity and cell inhibition mechanism of using Citrullus colocynthis on human glioblastoma U87 cell line was investigated.

The plant extract was extracted using ethanol (70%). To investigate the cytotoxic effects of Citrullus colocynthis extract on U87 cell line, 10 4 cells per well was incubated with different concentration of the prepared plant extract in groups for different concentrations during 24, 48, and 72 hours and the impact was measured using MTT assay. 24 hours IC50 was used for gene expression that was investigated using Real-time PCR.

From analytical data results, it was determined that at the concentrations higher than 1000 µg/ml, the plant extract can inhibit the cell growth completely and calculated IC50 for 24,48, and 72 hours incubation is 1337.42 (p≤0.0001), 892.99 (p=0.0012), and 387.49 (p=0.0001) µg/ml respectively. Casp3 and Casp8 gene expression in U87 cells, treated with IC50 concentration of plant extract for 24 hours, was 3.052-fold (P=0.0265) and 2.099-fold (P=0.0013) more than normal media incubated U87 cells, respectively.

The data revealed from this work determined that Citrullus colocynthis extract has concentration and time dependent toxicity effects on U87 cell line and the inhibition mechanism of this plant may be due to the induced Casp3 and Casp8 gene expression which leads to extrinsic apoptosis pathway. So, Citrullus colocynthis extract can be used to inhibit glioblastoma cells growth and may treat brain tumor.

Sesame seeds contain phytoestrogens and antioxidants that can affect the reproductive system. As the seminal vesicle is important in male reproductive activity, in the present study, effects of Sesame seeds consumption on histomorphometric structure of seminal vesicle in young adult rats were evaluated. 

In this study, 40 adult and young rats were divided into 4 groups. Each young and old control group, were divided into 2 groups (30 days and 60 days) and fed with pellet (28 grams) daily. The treatment group was divided into two groups (30 and 60 days old) and fed with 8.5 grams of Sesame seeds and 19.5 grams of pellet (30% and 70% of the diet) daily. At the end of study, rats were euthanased and seminal vesicle were collected. The tissue sections of samples were prepared using standard tissue sectioning (thickness of 5-6 μm) and stained with Hematoxylin-Eosin. Blood testosterone levels were measured too. 

Histomorphometric evaluation of seminal vesicle in young rats fed with sesame for 30 and 60 days showed that the thicknesses of the epithelial tissue, muscular and whole wall layer were significantly increased. In old rats fed for 30 and 60 days, the thickness of muscular layer and whole wall were significantly increased. Testosterone level was increased in young rats fed with Sesame. There were no significant effects in the structure of the seminal vesicle in older rats compared with young rats following sesame consumption.

Following age, a little tissue changes were seen in the seminal vesicle structure and it seems that Sesame can increasing the thickness of seminal epithelial tissue.

Breast cancer is the most common cancer among women. Given that plant compounds have been used to treat cancer for centuries. Despite some reports of antitumor effects on some species of this genus, the anticancer activity of Stachys setifera has not yet been reported. The aim of this study was to investigate the cytotoxic effect of hydroalcoholic extract of Stachys setifera on MCF-7 cancer cell line.

In this experimental study, MCF-7 cancer cells were cultured and then the cells were incubated with different concentrations of barbed spike hydralcholic extract and cisplatin for 24 hours. Cancer cell survival was measured using MTT assay. The effects of the extract and the tests performed on the mechanisms of cell death were stopped by flow cytometry. Cisplatin was used as a positive control in this study. The collected data were analyzed using one-way analysis of variance andTukeychrs post hoc test (p <0.05).

The IC50 results of hydroalcoholic extract Stachys setifera and cisplatin on MCF-7 cells after 24 hours were 827.52 and 4.34 μg / ml, respectively. The results showed that the inhibition of cell proliferation was dose-dependent. Hydroalcoholic extract Stachys setifera and cisplatin stops the growth of MCF-7 cells in the G0 / G1 phase. The percentage of cells in the extract control cells and cisplatin (42.94, 50.68 and 59.11).

Stachys setifera extract inhibits the proliferation of MCF-7 breast cancer cells in a dose-dependent manner and possibly enters the cells into the apoptotic phase. Therefore, the plant can be a good candidate to prevent the spread of breast cancer

Protein synthesis and degradation are dynamically regulated processes that to control the accretion or loss of muscle mass. However, the mechanisms responsible exercise-induced heart hypertrophy remains elusive. The aim of this study was to investigate the effect of aerobic training on expression of some indices of myocardial hypertrophy and atrophy in male rats.

In this experimental study, 16 Wistar male rats 10 weeks old with 299.74±19.32 g weight were randomly classified into control and training groups. Training group have performed an aerobic running program (at 10-18 m/min, 10-40 min/day, and 5 days/week) on a motor-driven treadmill for six weeks. At the end of six weeks heart tissue samples were collected and used for determination of gene expression (AMPK, FOXO3, MAFbx and MuRF1). Data were analyzed using Independent t test at p<0.05.

 Six weeks aerobic training significant increases heart AMPK in rats (p=0.014). Also heart FOXO3 (p=0.022), MAFbx (p=0.032) and MuRF1 (p=0.028) was significantly decrease than training group.

Aerobic exercise improves heart structure by improving the parameters that affect cardiac size, possibly improving cardiac function.

The  term  resistance  economy  was  first  introduced  by  Leader  of  Islamic Republic   of   Iran,   which   includes      components.  It  seems  that  using  resistance economy  is  associated  with  beneficial  effects  on  the  health  sector. this study is based on leadership guidelines for conducting studies on resistance economics focused  on  economic  problems  and  oppressive  sanctions  that  have  challenged  the management   of   hospitals   to   design   an   operational   model   for   implementing resistance   economics   in the shiraz medical  university   hospitals.

This  study  is  a  combined  research  that  was  conducted  in  several stages. Our  research  population  consisted  of  20  professors,  officials,  familiar  and experienced  managers  and  experts  aware  of  the  economic  issues  of  hospitals affiliated  to  shiraz University  of  Medical  Sciences. Targeted sampling method and snowball technique were used.  In the first stage, the study was performed by analyzing the current situation and conducting interviews. In the second stage, with the  help  of  interview  output,  using  the  Delphi  technique  bared  on  questionnaire, and MAXQDA and SPSS software were used to analyze the data.

Based on the content analysis of specialized interviews and review of the  current  situation,  34  indicators in 6 area(management  structure,  human  resources,  cost  management,  financing,  supply chain, scientific and research) were  identified. After performing three rounds of Delphi, 25 components with a score above 0.7 were confirmed. The criteria were then accepted using confirmatory factor analysis. Finally, in the structural equation model, the factor load of all the criteria greater than 0.3 and also their related t-statistic were obtained above 1.96 and the proposed model was confirmed.

Conclusion  : 

In   order   to   implement   resistance   economics   in   hospitals, the component of management  structure,  human  resources,  cost  management,  financing,  supply chain, scientific and research have priority, respectively. The components identified in this study can be a guide for policy makers and managers to overcome the economic problems of hospitals.

Among working women, health care professionals are more exposed to environmental factors and adverse working conditions than women working in other occupations. A large group of health professionals are nurses and operating room staff who provide services in hospitals. For this purpose, the present study was conducted to investigate the effect of adverse working conditions on the incidence of spontaneous abortion in the staff of operating room and nursing departments of Isfahan educational hospitals.

This descriptive study was conducted on 300 women working in nursing wards and operating room in educational hospitals in Isfahan from 2017 to 2018. The data collection tool was a questionnaire. The questionnaire collected demographic and fertility information, occupational status, and adverse pregnancy outcomes during the last pregnancy. Data were analyzed using SPSS software (version 16) and T-test, X2 and Mann-Whitney tests. P value less than 0.05 was considered significant.

In this study, 13 (8.7%) of women working in the operating room and 12 (8.1%) of women working in the nursing department had a history of spontaneous abortion in the last pregnancy, which was not statistically significant (p=0.85). 111 operating room staff (74%) stood for more than 3 hours, 42 (28.1%) worked more than 44 hours per week, and 57 (38%) experienced a restless environment with almost constant noise. The operating room group was relatively more exposed to anesthetic gases (98%), sterilizing agents (94%) and x-rays (97%), which was statistically significant (p <0.05). Despite differences in most adverse work situations, there was no significant difference in spontaneous abortion between the two groups (p = 0.85).

Regarding the frequency of undesirable situations in both groups, especially the operation room group, there was no significant difference in the incidence of spontaneous abortion in the two groups. Therefore, it is suggested that a similar study should be carried out with a larger number of samples and, if possible, at national level and as a prospective cohort.

Finding an efficient biomarker or combination of them as predictors of early-onset neonatal sepsis is very important. On the other hand, the use of umbilical cord blood samples in laboratory tests, along with its simplicity and availability, reflects neonate metabolic status.

The aim of this study was to investigate the association between umbilical cord blood laboratory findings with early onset neonatal sepsis.

In this cross-sectional analytical study, we evaluated 180 neonates, 90 infants susceptible to early onset sepsis, and 90 healthy neonates who were admitted in neonatal and  neonatal intensive care unit wards, between January 2015 and 2016   at Kosar Hospital. The data from these participants were obtained by a check list including  gender,  birth weight, gestational age, white blood cell count, neutrophil counts, erythrocyte sedimentation rate (ESR), and reactive protein C (CRP) levels. Both descriptive and statistical analysis methods were applied. P value less than  0.05, statistically was significant.

Mean of ESR and neutrophil count in cord blood sample of suspected sepsis neonates was higher than well baby.  The mean of CRP levels in cord blood sample of suspected sepsis neonates slightly was higher than well baby. But there was no statistically significant difference between the CRP levels in both groups.

The results of this study showed that  the laboratory findings used in combination with each other as an early predictor of neonatal sepsis was  reliable.

Increasing food consumption outdoors in different societies has raised the risk of transmission of foodborne pathogens as a global health problem. Molecular typing methods such as REP-PCR produced DNA profiles for differentiation and characterization of pathogenic strains. The aim of this study was to evaluate the accuracy of molecular fingerprinting method based on repeated sequences (rep-PCR) to determine the affinities between different of Enterococcus fascia isolated from beef meat sold in Shahrekord retail markets.

In this descriptive cross-sectional study, 80 meat samples were examined by biochemical and molecular methods for the presence of Enterococcus fascia and the molecular patterns were determined on the basis of the existence or absence of DNA fragments were separated according to their size by agarose gel electrophoresis. By using a computer program NTSYS version2.02e, dendrogram were drawn.

Of the 80 samples, 66 (82.5%) were identified as Enterococcus, while 30 (45.45%) were Enterococcus fascia. Based on Genetic Classification by Rep-PCR, 30 isolates of Enterococcus fascia were included in 18 profiles. Placement of the studied isolates in several subgroups showed the acceptable discrimination power of Rep-PCR technique in genotyping of Enterococcus fascia.

 The results of this study showed that Rep-PCR is a simple, fast, and method with high dispersal ability to characterize the genetic diversity of Enterococcus fascia strains.

Crime and criminal behavior can occur under the influence of various environmental and personality factors. One of the personality traits that is especially important in adolescents is the level of arousal and reaching the optimal level of arousal, which may pose risks for them and is considered as an accusation and ultimately a crime. The aim of this study was to determine the excitement of children and adolescents in Yasuj and its relationship with crime.

This study is a descriptive-analytical and comparative study, which was performed on a sample of 160 children and adolescents of the Correctional Center, Welfare Organization and Yasuj schools in two groups of 80 people (case, control), by sample method. Objective sampling was selected based on purpose and evaluated with demographic and excitement questionnaires of Rousseau children and adolescents. Data were analyzed using SPSS software version 23.

The results showed that the age of the sample group was between 10 and 18 years and their highest crime was theft (41.2%) and conflict (32.6%). In addition, there was a significant difference between children and adolescentschr) excitement with crime (P <0.05), and there was a significant relationship between excitement components such as excitement, adventure and social deterrence, children and adolescents and crime (P <0.05). 0.05), was observed.

Chest radiography is one of the most common X-ray imaging procedures performed worldwide. During this process, in addition to the chest, other tissues, including the thyroid, are also exposed to radiation. Due to the fact that one of the most important risk factors for thyroid cancer is ionizing radiation, measuring the absorption of X- rays in the thyroid is of special importance.

 Energies of 100, 70 and 80 kV were used in the Monte Carlo code. 300 million particles were used in the BEAMnrc simulation and 100 million particles in the DOSXYXnrc simulationThen, using chest CT scan images obtained from patients, the chest phantom was designed by CTCREATE method. The dose absorbed in the thyroid gland was calculated using BEAMdp from the output obtained from BEAMnrc.

D / Dmax was obtained in different coordinates from 0.54 to 6.91 and the calculation error was calculated from 0.32 to 18.73%.

Chest radiography can lead to high dose absorption in some areas of thyroid tissue. Dose absorption in thyroid is present in all energy ranges, which is typically done on chest radiographs.